Evolution of the Germ Layers
Hashimshony T, Feder M, Levin M, Hall BK, Yanai I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature. 2015 519:219-22. Editor’s summary: The germ-layer theory — which holds that all the cells and tissues of the body can be grouped into three fundamental layers — goes back to the roots of developmental biology…
The mid-developmental transition
Levin M, Anavy L, Cole AG, Winter E, Mostov N, Khair S, Senderovich N, Kovalev E, Silver DH, Feder M, Fernandez-Valverde SL, Nakanishi N, Simmons D, Simakov O, Larsson T, Liu SY, Jerafi-Vider A, Yaniv K, Ryan JF, Martindale MQ, Rink JC, Arendt D, Degnan SM, Degnan BM, Hashimshony T, Yanai I. The mid-developmental transition and…
CEL-Seq2: Single-cell RNA-Seq
In 2012, our lab published CEL-Seq, a method for single-cell RNA-Seq. The method is highly-multiplexed, uses in vitro transcription (IVT) to amplify, and has become one of the best methods for single-cell RNA-Seq. We believe it is actually THE best method! Here is the CEL-Seq protocol. This is how we freeze individual cells, though we also use a…
The Society of Genes
Here’s the website for the book: thesocietyofgenes.org Nearly four decades ago Richard Dawkins published The Selfish Gene, famously reducing humans to “survival machines” whose sole purpose was to preserve “the selfish molecules known as genes.” How these selfish genes work together to construct the organism, however, remained a mystery. Standing atop a wealth of new research, The…
yanailab.org 